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800.334.5551

DNALC Forensic DNA Fingerprinting Kit with CarolinaBLU® (with perishables)

$164.70
(backordered: in stock 8/11/22)

Description

Advanced—For experienced high school and college classes; requires some technical skill.

Developed in cooperation with the DNA Learning Center, this advanced lab uses plasmid isolation and restriction analysis to illustrate forensic DNA typing. Students are provided with 2 samples of E. coli cells to represent blood samples from 2 suspects. Plasmid DNA is extracted using a miniprep procedure and cut with restriction enzymes, along with a DNA sample representing the evidence from a crime scene. The resulting "DNA fingerprints" of the suspects are compared for a match with the evidence. Materials are sufficient for 8 groups of 4 students. Foreign orders require special handling. Comes with perishables.

FREE 1-year access to digital resources that support NGSS instruction included.

Specifications

Shipping Information or Purchase Restrictions
  • Exclamation icon Excepted Quantity - This product is/contains a DOT regulated hazardous material in an excepted quantity. This does not incur a hazardous materials fee. Orders shipping to HI and AK are subject to review and may incur additional shipping charges. This item contains living or perishable material and ships via 2nd Day or Overnight delivery.
What’s Included:
  • E. coli Slant Cultures 
  • 1 Restriction Buffer, 180 µL 
  • 1 Restriction Enzyme Mixture, 85 µL 
  • 1 RNase A, 250 µL, 5 µL/mL 
  • 1 Evidence A DNA, 80 µL, 0.1 µg/µL 
  • 1 Evidence B DNA, 80 µL, 0.1 µg/µL 
  • 4 Luria Broth, 10 mL
  • 2 Glucose-Tris-EDTA, 3.5 mL
  • 2 Potassium Acetate-Acetic Acid Solution, 3.5 mL
  • 2 Isopropanol, 100%, 10 mL
  • 1 Ethanol, 95%, 15 mL
  • 1 Tris-EDTA Buffer, 3.5 mL
  • 1 Sodium Dodecyl Sulfate Solution, 10%, 1 mL
  • 1 0.22 N Sodium Hydroxide Solution, 8 mL
  • 1 Loading Dye, 1 mL
  • 1 TBE Electrophoresis Buffer, 20x, 200 mL
  • 1 Agarose, 5 g
  • 1 Sterile Distilled Water, 1 mL
  • CarolinaBLU® Final Stain, 400 mL
  • CarolinaBLU® Gel and Buffer Stain, 14 mL
  • 130 Microcentrifuge Tubes, 1.5 mL
  • 8 Staining Trays
  • 100 Transfer Pipets
  • 16 Disposable Gloves
  • 4 Inoculating Loops
  • 1 Teacher’s Manual with Reproducible Student Guide
  • expand to see full list
Needed But Not Included:
  •  Document Camera or Overhead Projector
  •  Water Bath, 37° C
  •  Water Bath, 60° C (optional)
  •  Microcentrifuge (that generates at least 2,300 x g)
  •  Gel Electrophoresis Chambers (with casting trays and well-forming combs)
  •  Electrophoresis Power Supplies
  •  White Light Transilluminator (for viewing gels)
  •  Micropipets (for 1- to 1,000-µL volumes)
  •  Masking Tape (for sealing gel casting tray)
  • 8 Racks (for 1.5-mL centrifuge tubes)
  •  Flask or Beaker, 1 L (for agarose)
  • 8 Beakers or Similar Containers, 600 mL (for ice)
  • 8 Beakers or Similar Containers, 600 mL (for waste, such as used pipets)
  •  Carboy or Container, 4 L or Larger (for electrophoresis buffer)
  •  Ice (preferably crushed)
  •  Distilled Water
  •  Permanent Markers
  •  Boiling Water Bath or Microwave Oven
  •  Autoclave or 10% Household Bleach Solution (for decontamination of bacterial waste before disposal)
  •  Disinfectant (10% household bleach solution, 70% ethanol, isopropanol, or other for cleaning lab surfaces)
  •  Paper Towels
  •  Camera (for recording results, optional)
  • expand to see full list
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