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This lab uses plasmid isolation and restriction analysis to illustrate forensic DNA typing. Students are given 2 samples of E. coli cells to represent blood samples from 2 suspects. Plasmid DNA is extracted using a miniprep procedure and cut with restriction enzymes, along with a DNA sample representing the evidence from a crime scene. Students perform gel electrophoresis of the digested DNA and compare the fragment patterns to determine which suspect's DNA fingerprint matches the evidence.

Product Details
Advanced—For experienced high school and college classes; requires some technical skill.
Illustrate principles of forensic DNA typing by creating and analyzing DNA fingerprints

This lab uses plasmid isolation and restriction analysis to illustrate forensic DNA typing. Students are provided with 2 samples of E. coli cells to represent blood samples from 2 suspects. Plasmid DNA is extracted using a miniprep procedure and cut with restriction enzymes, along with a DNA sample representing the evidence from a crime scene. Students perform gel electrophoresis of the digested DNA and compare the fragment patterns to determine which suspect's DNA fingerprint matches the evidence.

Product Features

• Students isolate DNA and perform restriction digest and electrophoresis to produce DNA fingerprints
• Materials are sufficient for 8 groups of 4 students (32 students total)
• Safe-to-use CarolinaBLU® DNA stain produces blue bands
• Includes voucher to request delivery of the perishable materials at a later date
• Perishable materials can be replenished with the refill kit (item #211205C)
• Included paper model activity simulates DNA STR digest and analysis
• A complete visual lab procedure complements the written protocol
• Resource section connects the kit activities to authentic lab techniques, real-world careers, historical perspectives, and more
• Kit content and the skills used by students during the investigations support obtainment of BCSI biotechnology micro-credentials
• Kit provides an NGSS phenomena activity and aligns to science and engineering practices and crosscutting concepts

Note: Kit includes a voucher to request perishable materials later at your convenience. Contact Carolina or return the voucher to request delivery of perishables.

Time Requirement
Four 60- to 90-minute class periods.

Digital Resources
Includes 1-year access to digital resources that support instruction. Digital resources may include a teacher manual and student guide, pre-lab activities and setup videos, simulations, and post-lab analysis and assessments.

Learning Objectives
Students will understand:

• Genetic variation between individuals of a species
• Use of DNA fingerprinting to identify individuals

Methods for Biological Research Used in the Kit

• Perform DNA minipreps to isolate DNA from bacteria
• Perform restriction digests and gel electrophoresis
• Analyze DNA banding patterns to match fingerprints from suspects and evidence

Connection to the Next Generation Science Standards*
Science and Engineering Practices

• Engaging in Argument from Evidence
• Analyzing and Interpreting Data

Disciplinary Core Ideas

• LS1.A: Structure and Function
• LS3.A: Inheritance of Traits
• LS3.B: Variation of Traits

Crosscutting Concepts

• Patterns

*Next Generation Science Standards® is a registered trademark of WestEd. Neither WestEd nor the lead states and partners that developed the Next Generation Science Standards were involved in the production of this product, and do not endorse it.