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Description
Students set up their own restriction digests of lambda DNA with BamHI, EcoRI, and HindIII using Instant DNA and Instant Enzymes. They then separate the resulting DNA fragments by electrophoresis. Gels can be run in 3-1/2 hours using three 9-V batteries or overnight with 1 battery. The Classroom Kit contains instructions and 5 sets of apparatus (with 4-well comb) with enough materials for 10 separate experiments.
Intermediate - Easy to perform; requires some background knowledge.
Exploring restriction analysis and electrophoresis of DNA classroom kit. Students set up their own restriction digests of lambda DNA with BamHI, EcoRI, and HindIII using Instant DNA and Instant Enzymes. They then separate the resulting DNA fragments by electrophoresis. Gels can be run in 3-1/2 hours using three 9-V batteries or overnight with 1 battery. The Classroom Kit contains instructions and 5 sets of apparatus (with 4-well comb) with enough materials for 10 separate experiments.
