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Intermediate - Easy to perform; requires some background knowledge.
Students set up their own restriction digests of lambda DNA with BamHI, EcoRI, and HindIII using Instant DNA and Instant Enzymes. They then separate the resulting DNA fragments by electrophoresis. Gels can be run in 3-1/2 hours using three 9-V batteries or overnight with one battery. Provides enough supplies for 2 demonstrations.
Intermediate - Easy to perform; requires some background knowledge.
Exploring restriction analysis and electrophoresis of DNA demonstration kit. Students set up their own restriction digests of lambda DNA with BamHI, EcoRI, and HindIII using Instant DNA and Instant Enzymes. They then separate the resulting DNA fragments by electrophoresis. Gels can be run in 3-1/2 hours using three 9-V batteries or overnight with one battery. This Demonstration Kit contains instructions, 1 set of apparatus (with 4-well comb), and sufficient materials for 2 demonstrations.