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PCR Forensics Simulation 8-Station Kit

$167.55
Product Highlights
  • Create DNA fingerprints and simulate how forensic investigators solve crimes
  • Introduce DNA fingerprinting and PCR (simulated; no thermocycler required)
  • Kit Refill, bulk DNA, and digital resources available separately
(in stock)

Description

Product Details
Intermediate—Easy to perform; requires some background knowledge.

Simulate the use of DNA in forensic investigations with this kit that has students electrophorese DNA samples from a fictional crime scene and 2 suspects. The DNA samples simulate the results of polymerase chain reaction, providing opportunities to teach and reinforce that concept. Upon completing the electrophoresis, students use their simulated DNA fingerprint to determine which suspect (if either) is the guilty party.

Product Features

  • DNA fingerprints of 2 suspects are compared at 2 loci
  • Uses CarolinaBLU® stain
  • Kit includes materials for 8 student stations

Time Requirement
Teacher prep, 30 min before lab. Two to three 50-minute class periods.

Digital Resources
Includes 1-year access to digital resources that include a teacher’s manual, student guide, and more.

Learning Objectives

  • Learn about polymerase chain reaction and DNA fingerprinting.
  • Perform gel electrophoresis, a basic technique for separating DNA fragments by size.
  • Learn about real-world applications of PCR.

Prerequisite Knowledge and Skills
Students should have some understanding of DNA structure and function, DNA fingerprinting, polymerase chain reaction (PCR), and gel electrophoresis.

Specifications

What’s Included:
  • 8 Suspect X-1 DNA, 20 µL
  • 8 Suspect X-2 DNA, 20 µL
  • 8 Suspect Y-1 DNA, 20 µL
  • 8 Suspect Y-2 DNA, 20 µL
  • 8 Evidence 1 DNA, 20 µL
  • 8 Evidence 2 DNA, 20 µL
  • 16 Gloves
  • 1 Agarose, 6 g
  • CarolinaBLU® Final Stain, 250 mL
  • CarolinaBLU® Gel and Buffer Stain, 7 mL
  • 8 Staining Trays
  • 56 Pipets
  • 1 TBE Buffer, 20x, 200 mL
  • 1 Teacher’s Manual and Reproducible Student Guide
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Needed But Not Included:
  • 8 Gel Electrophoresis Chambers
  • 8 Power Supplies
  • 8 Racks (for samples)
  • 1 Graduated Cylinder, 1 L
  • 1 Flask, 4 L
  • 1 Flask, 1 L
  • 1 Water Bath, Hot Plate, or Microwave
  • 8 Permanent Markers
  •  PPE for Instructor and Students
  •  White-light Box, Slide Viewer, or Overhead Projector
  •  Aluminum Foil
  •  Masking Tape
  •  Distilled or Deionized Water
  •  Microcentrifuge (optional)
  •  Funnel (optional)
  •  Instant or Digital Camera (optional)
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