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Electrophoresis of precut DNA with an analytical application. Students cast gels and perform electrophoresis of ready-to-load aliquoted samples of predigested plasmid DNA. After staining with CarolinaBLU® stain, students determine the sizes of the plasmid DNA fragments in each sample and use the data to deduce a restriction map of the plasmid.
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Advanced - For experienced high school and college classes; requires some technical skill.
Electrophoresis of precut DNA with an analytical application. Students cast gels and perform electrophoresis of ready-to-load aliquoted samples of predigested plasmid DNA. After staining with CarolinaBLU® stain, students determine the sizes of the plasmid DNA fragments in each sample and use the data to deduce a restriction map of the plasmid. Kit contains sufficient materials for 4 teams of students to perform the exercise.
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