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800.334.5551

Detecting Epigenetic DNA Methylation in Arabidopsis Amplification Kit (with perishables)

$290.10
(in stock)

Description

Advanced—For experienced high school and college classes; requires some technical skill.

  • Introduce students to the growing field of epigenetics
  • Gain experience working with the model plant Arabidopsis thaliana
  • Investigate the role of DNA methylation in gene regulation
  • Use a protocol designed especially for the classroom
Utilize methylation-sensitive enzymes to explore epigenetics—heritable changes in gene expression—that affect flowering in Arabidopsis. By growing wild-type Ler and mutant fwa-1 plants, students are able to observe phenotypic differences in flowering and then investigate this difference using multiple molecular techniques. Students conduct DNA extraction, restriction enzyme digest, PCR, gel electrophoresis, and bioinformatics to investigate the pivotal role DNA methylation plays in gene regulation. Kit contains materials for 25 DNA extractions and PCR reactions. Note: Kit includes perishable materials. Kit must be used within 4 weeks of receiving it.

Specifications

Shipping Information or Purchase Restrictions
  • Exclamation icon This item contains living or perishable material and ships via 2nd Day or Overnight delivery.
What’s Included:
  • Arabidopsis Seed Pack from Ler (Landsberg erecta) Wild Type 
  • Arabidopsis Seed Pack from fwa-1 (flowering wageningen) Mutant 
  • FWA Primer/Loading Dye Mix, 700 µL 
  • 1 McrBc Buffer, 10x, 200 µL 
  • 1 McrBc Enzyme, 10 U/µL, 20 µL 
  • 1 Guanosine 5′–Triphosphate (GTP), 100 mM, 15 µL 
  • 1 BSA, 10 mg/mL, 30 µL 
  • 1 Sterile Distilled Water, 1 mL 
  • 1 RNAse Solution, 5 mg/mL, 250 µL 
  • 1 Potting Soil, Bag 
  • 1 pBR322/BstNI Markers, 0.075 µg/µL, 130 µL 
  • 25 Ready-to-Go™ PCR Beads, 0.2-mL Tubes 
  • 1 Nuclei Lysis Solution, 12 mL
  • 1 Protein Precipitation Solution, 3.5 mL
  • 2 Isopropanol, 100%, 20 mL
  • 1 Ethanol, 70%, 30 mL
  • 1 DNA Rehydration Buffer, 2.5 mL
  • 15 Plastic Pestles
  • 1 Mineral Oil, 5 mL
  • 1 Planting Flat and Tray
  • 1 Plastic Dome Lid
  • 1 Teacher’s Manual with Reproducible Student Guide
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Needed But Not Included:
  •  Tweezers, Scissors, or Scalpel
  •  Water Baths/Heating Blocks (65° C and 37°l C)
  •  Micropipets and Tips, 1 to 1,000 µL
  •  Microcentrifuge
  • 32 Microcentrifuge Tubes, 1.5 mL
  • 8 Microcentrifuge Tube Racks
  • 1 Thermal Cycler, Programmable
  •  Electrophoresis Chambers
  •  Electrophoresis Power Supplies
  •  Container with Ice
  •  Computer with Internet Access
  •  Microwave, Hot Plate, or Boiling Water
  •  Vortexer (optional)
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