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800.334.5551

Human Alu DNA Extraction, Amplification, and Electrophoresis Kit with Ethidium Bromide and 0.2-mL Tubes (with voucher)

$224.30
(in stock)

Description

Advanced—For experienced high school and college classes; requires some technical skill.

  • Simple, robust protocol is ideal for classroom use
  • Incorporates Hardy-Weinberg equilibrium and chi-square analysis
  • Helps demonstrate real-world applications of PCR
  • Discover how Alu insertions are used to track world population migrations
  • CD-ROM contains animations and video clips for classroom instruction

Students use safe saline mouthwash or hair and Chelex® extraction to obtain a sample of their own DNA. Then they use polymerase chain reaction (PCR) to amplify a 300-nucleotide Alu insertion into an intron of the H-cadherin gene. Gel electrophoresis separates the 2 alleles, and each student determines his/her own genotype. The experiment provides an introduction to human population genetics as participants learn to score genotypes and calculate genotype and allele frequencies.

Kit contains instructions, a CD-ROM, and materials for 25 students to perform DNA extraction, amplification, and electrophoresis (staining with ethidium bromide). Note: Kit includes a voucher to request perishable materials later at your convenience. Contact us or return the voucher to request delivery of perishable materials.

   This item is sold and shipped to schools and businesses only. Not available to individuals or residential addresses. For substitution suggestions, please call us at 800.334.5551 (8am to 8pm, ET, Monday-Friday).

Specifications

Shipping Information or Purchase Restrictions
  • Exclamation icon This item is sold and shipped to schools and businesses only. Not available to individuals or residential addresses. Visit our Login & Registration page to learn how to create a Login and link it to an approved school or business account.
What’s Included:
  • 1 PV92B Primer/Loading Dye Mix, 700 µL
  • 1 pBr322/B s t NI Markers, 0.075 µg/µL, 130 µL
  • 1 Proteinase K, 100 µg/mL, 5 mL
  • 1 Chelex® Resin, 0.5 g
  • 25 PCR Tubes Containing Ready-to-Go-Beads™ (0.2-mL capacity)
  • 1 Mineral Oil, 5 mL
  • 1 Agarose, 5 g
  • 1 TBE, 150 mL
  • 1 Ethidium Bromide, 1 µg/mL, 250 mL
  • 4 Latex Gloves
  • 6 Staining Trays
  • Alu CD-ROM
  • 1 Teacher's Manual with Reproducible Student Guide
  • expand to see full list
Needed But Not Included:
  •  Saline Solution, 0.9% (10 mL per student)
  •  Micropipets and Tips (1 µL to 1,000 µL)
  •  Microcentrifuge Tubes, 1.5 mL (2 per student)
  •  Microcentrifuge Tube Racks
  •  Microcentrifuge for 1.5-mL Tubes
  •  PCR Tubes, 0.2 mL or 0.5 mL (1 per student)
  •  Tube Adapters (for microcentrifuge)
  •  Thermal Cycler
  •  Electrophoresis Chambers
  •  Electrophoresis Power Supplies
  •  Gel Staining Trays
  •  UV Transilluminator (for ethidium bromide stain)
  •  Paper Cup (1 per student)
  •  Permanent Markers
  •  Container of Crushed Ice
  •  Camera (optional)
  •  Boiling Water Bath (optional)
  • expand to see full list
Return Policy:

This product is backed by our 100% Satisfaction Guarantee. If for any reason you are not satisfied with any item, you may return it for a replacement, a refund, or credit. Read more about our Return and Exchange Policy  »

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