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This experiment introduces human population genetics by students performing PCR analysis of their own genotypes. Students use safe saline mouthwash and Chelex® extraction to obtain their own DNA sample. They then use polymerase chain reaction (PCR) to amplify a 300-nucleotide Alu insertion into an intron of the H-cadherin gene. Each student scores their genotype, and the compiled class results are used as a case study in human population genetics. Kit includes enough materials for 25 students.

Product Details
Advanced—For experienced high school and college classes; requires some technical skill.
Discover how Alu insertions are used to track world population migrations with modern biotechnology techniques

This experiment introduces human population genetics by students performing PCR analysis of their own genotypes. Students use safe saline mouthwash and Chelex® extraction to obtain their own DNA sample. They then use polymerase chain reaction (PCR) to amplify a 300-nucleotide Alu insertion into an intron of the H-cadherin gene. Each student scores their genotype, and the compiled class results are used as a case study in human population genetics. Tools for testing Hardy-Weinberg equilibrium, comparing the PV92 insertion in world populations, and simulating the inheritance of a new Alu insertion are explored using DNALC BioServers.

Product Features

• DNA extraction, isolation, and amplification by PCR only
• Sufficient materials for 25 DNA extractions and PCR reactions
• Includes perishable materials
• Included paper model activity simulates cycles of polymerase chain reactions
• Complete visual lab procedure complements written protocol
• Resource section connects the kit activities to authentic lab techniques, real-world careers, historical perspectives, and more
• Kit content and the skills used by students during the investigations support obtainment of BCSI Biotechnology micro credentials
• Kit provides an NGSS phenomena activity and aligns to science and engineering practices and crosscutting concepts
• Additional Alu PV92 primer set available (item #211502)

Note: Kit includes the perishable materials when shipped.

Time Requirements
The laboratory activities will take 3 to 4 days to complete using 60- to 90-minute class periods.

Digital Resources
Includes 1-year access to digital resources that support instruction. Digital resources may include a teacher manual and student guide, pre-lab activities and setup videos, simulations, and post-lab analysis and assessments.

Learning Objectives
Students will understand:

• How to collect and analyze genetic information in populations
• The use of allele and genotype frequencies to test Hardy-Weinberg equilibrium
• The use of DNA polymorphisms in the study of human evolution
• Identity by descent from a common ancestor
• The movement between in vitro experimentation and in silico computation

Methods for Biological Research Used in the Kit

• DNA extraction and purification
• Polymerase chain reaction (PCR)
• Bioinformatics

Connection to the Next Generation Science Standards*
Science and Engineering Practices

• Analyzing and Interpreting Data

Crosscutting Concepts

• Structure and Function
• Patterns

*Next Generation Science Standards® is a registered trademark of WestEd. Neither WestEd nor the lead states and partners that developed the Next Generation Science Standards were involved in the production of this product, and do not endorse it.