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Description
Introduces human population genetics by students performing PCR analysis of their own genotypes. Students use saline mouthwash and Chelex® extraction to obtain their own DNA sample, then use PCR to amplify a 300-nucleotide Alu insertion into an intron of the H-cadherin gene. Gel electrophoresis separates the 2 alleles. Each student scores their genotype, and compiled class results are used as a case study in human population genetics. Includes materials for 25 students.
Advanced—For experienced high school and college classes; requires some technical skill.
Discover how Alu insertions are used to track world population migrations with modern biotechnology techniques
This experiment introduces human population genetics by students performing PCR analysis of their own genotypes. Students use safe saline mouthwash and Chelex® extraction to obtain their own DNA sample. They then use polymerase chain reaction (PCR) to amplify a 300-nucleotide Alu insertion into an intron of the H-cadherin gene. Gel electrophoresis separates the 2 alleles. Each student scores their genotype, and the compiled class results are used as a case study in human population genetics. Tools for testing Hardy-Weinberg equilibrium, comparing the PV92 insertion in world populations, and simulating the inheritance of a new Alu insertion are explored using DNALC BioServers. Kit includes enough materials for 25 students.
