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Using Highly Variable Polymorphisms in Forensic Biology and Population Genetics DNA Extraction, Amplification, and Electrophoresis Kit with CarolinaBLU® and 0.2-mL Tubes (with perishables)

$227.70
(in stock)

Description

Advanced—For experienced high school and college classes; requires some technical skill.

Introduce students to Hardy-Weinberg equilibrium and the concept of allele frequencies in populations

  • Provides an introduction to the use of PCR in forensics
  • Helps demonstrate real-world applications of PCR

This lab illustrates the use of DNA typing to identify individuals in court cases and disasters. It assays for variable numbers of tandem repeats (VNTR) polymorphisms, which are caused by short, repeated copies of a 16-nucleotide sequence at the pMCT118 locus. Differences in the number of repeated units produce longer and shorter alleles, which can be resolved by gel electrophoresis. Because the VNTR locus has more than 29 different alleles, a panel of student types shows a variety of different genotypes.

Use this kit to teach population genetics, Hardy-Weinberg equilibrium, and forensics techniques. The repeated structure of the VNTR polymorphism makes it more difficult to amplify, so this experiment greatly benefits from a "hot start," in which 1 of the reagents is added during the first denaturing cycle.

Kit contains instructions and materials for 25 students to perform the DNA extraction, amplification, and electrophoresis (staining with CarolinaBLU®). Note: Kit is shipped with perishable materials.

Specifications

Shipping Information or Purchase Restrictions
  • Exclamation icon This item contains living or perishable material and ships via 2nd Day or Overnight delivery.
What’s Included:
  • 1 pMCT118 Primer/Loading Dye Mix, 700 µL 
  • 1 pBr322/B s t NI Markers, 0.075 µg/µL, 130 µL 
  • 1 Proteinase K, 100 µg/mL, 5 mL 
  • 1 Chelex® Resin, 0.5 g
  • 1 Mineral Oil, 5 mL
  • 25 PCR Tubes Containing Ready-to-Go-Beads™ (0.2-mL capacity)
  • 1 Agarose, 5 g
  • 1 TBE, 150 mL
  • CarolinaBLU® Final Stain, 250 mL
  • CarolinaBLU® Gel and Buffer Stain, 7 mL
  • 4 Latex Gloves
  • 6 Staining Trays
  • 1 Teacher's Manual with Reproducible Student Guide
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Needed But Not Included:
  •  Saline Solution, 0.9% (10 mL per student)
  •  Micropipets and Tips (1 µL to 1,000 µL)
  •  Microcentrifuge Tubes, 1.5 mL (2 per student)
  •  Microcentrifuge Tube Racks
  •  Microcentrifuge for 1.5-mL Tubes
  •  PCR Tubes, 0.2 mL or 0.5 mL (1 per student)
  •  Tube Adapters (for microcentrifuge)
  •  Thermal Cycler
  •  Electrophoresis Chambers
  •  Electrophoresis Power Supplies
  •  Gel Staining Trays
  •  White Light Box (for CarolinaBLU® stain)
  •  Paper Cup (1 per student)
  •  Permanent Markers
  •  Container of Crushed Ice
  •  Camera (optional)
  •  Boiling Water Bath (optional)
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