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BeginningEasy to perform; requires no experience in microbiology.
For centuries, spices have been used to preserve foods because they contain natural substances that act as inhibitors. Students grow 5 food spoilage microbes on black pepper, clove, and chili powder agars and examine the inhibitory effect of these common spices on the microbes' growth and/or sporulation. Kit materials are sufficient for 30 students working in groups of 3.
BeginningEasy to perform; requires no experience in microbiology.
For centuries, spices have been used to preserve foods because they contain natural substances that act as inhibitors. Students grow 5 food spoilage microbes on black pepper, clove, and chili powder agars and examine the inhibitory effect of these common spices on the microbes' growth and/or sporulation. Application to food spoilage prevention and the history of the spice trade is discussed and can be easily integrated into a multidisciplinary educational approach. Students practice aseptic technique and microbe culturing.
Time requirements:
Kit materials are sufficient for 30 students working in groups of 3. Kit ships with perishables included. Note: Keep bacterial and fungal cultures at room temperature; do not incubate or refrigerate.
Components | Qty | Included? |
---|---|---|
Bacillus cereus Plate Culture ![]() |
1 | Included |
Escherichia coli Plate Culture ![]() |
1 | Included |
Penicillium notatum Plate Culture ![]() |
1 | Included |
Pseudomonas fluorescens Plate Culture ![]() |
1 | Included |
Saccharomyces cerevisiae Plate Culture ![]() |
1 | Included |
Plate Count Agar, 125 mL | 1 | Included |
Black Pepper Agar, 125 mL | 1 | Included |
Chili Powder Agar, 125 mL | 1 | Included |
Cloves Agar, 125 mL | 1 | Included |
Sterile Petri Dishes, 100 × 15 mL | 50 | Included |
Sterile Applicator Sticks | 10 | Included |
Autoclavable Disposal Bag | 1 | Included |
Teacher’s Manual with Reproducible Student Guide | 1 | Included |
Isopropyl Alcohol, 70% | 1 | Needed, Not Included |
Waste Containers | 10 | Needed, Not Included |
Permanent Markers | 10 | Needed, Not Included |
Bunsen Burners (or alcohol burners) | 10 | Needed, Not Included |
Hot Water Bath | 1 | Needed, Not Included |
Microwave (optional) | 1 | Needed, Not Included |
Autoclave | 1 | Needed, Not Included |