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  • Advanced Bacterial Conjugation Kit (with perishables)

Advanced Bacterial Conjugation Kit (with perishables)

Item # 211127P

Teach your students about gene transfer between bacterial strains. In 1 of the included strains of E. coli, the gene for antibiotic resistance for streptomycin is located on the chromosome. In the other, the gene for antibiotic resistance for ampicillin occurs as extrachromosomal plasmid DNA. Students use streaking to determine which bacterial strain is the donor strain. Materials for 16 to 32 students (8 stations).
Price

$132.20

In stock and available to ship.
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Overview

Intermediate—Easy to perform; requires some background knowledge.

  • Designed for 16 to 32 students (8 stations)
  • Minimal teacher preparation
  • Inquiry-based learning

Teach your students about gene transfer between bacterial strains. In 1 of the included strains of E. coli, the gene for antibiotic resistance for streptomycin is located on the chromosome. In the other, the gene for antibiotic resistance for ampicillin occurs as extrachromosomal plasmid DNA. Students use streaking to determine which bacterial strain is the donor strain. Kit contains perishable materials.

Note: Antibiotic solutions and cultures last 1 month when refrigerated.
  • This item contains living or perishable material and ships via 2nd Day or Overnight delivery to arrive on a date you specify during Checkout. To ensure freshness during shipping, a Living Materials Fee may apply to orders containing these items.

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What's Included

This item ships with living or perishable materials (all   items). Please select a delivery date during Checkout.

Components Qty Included?
E. coli Strain I (Str)  1 Included
E. coli Strain II (Amp and Nal)  1 Included
Naladixic Acid Solution, 0.5%, 4 mL  1 Included
Streptomycin Solution, 2%, 4 mL  3 Included
Ampicillin Solution, 1%, 4 mL  3 Included
Luria Broth, 10 mL 8 Included
Luria Broth Agar, 400 mL 5 Included
Sterile Culture Plates 85 Included
Sterile Loops 100 Included
Digital Resource Instruction Card 1 Included
Teacher’s Manual and Reproducible Student Guide 1 Included
Laboratory Markers 8 Needed, Not Included
Incubator, 37° C (optional) 1 Needed, Not Included
Boiling Water Bath (or microwave) 1 Needed, Not Included
Masking Tape Needed, Not Included
Biohazard Disposable Bags Needed, Not Included
Solution of Household Bleach, 10% Needed, Not Included
Parafilm® or Plastic Wrap Needed, Not Included

Resources

Life Science

Living Organism Care Information

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