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Green Gene Colony Transformation 4-Station Kit (with perishables)

$77.45
(in stock)

Description

An eye-catching example of the genetic basis of inheritance

  • Green color is clearly visible in normal classroom light
  • No UV lighting needed
  • Pre-aliquoted and prepared reagents minimize teacher prep time
Designed to match traditional AP® Biology Lab 6. Open your students' eyes to the genetic basis of inheritance with this simple lab. Developed in cooperation with the DNA Learning Center, this kit clearly demonstrates the induced uptake of a jellyfish gene by E. coli. Students observe the phenotypic effect of adding the gene for GFP (green fluorescent protein). GFP is expressed on plain LB—no color development substrate or induction reagent is needed. Your students can easily view the "green" effects in normal classroom lighting. Colonies will fluoresce under UV light. The plasmid containing the GFP gene also contains an ampicillin resistance gene, so ampicillin can be used to select for transformants. Lab procedure is easy to follow. The 4-Station Kit includes enough materials for 4 teams of students. Includes perishable materials when kit is shipped.

AP® is a trademark registered and/or owned by the College Board, which was not involved in the production of, and does not endorse, this product.

Specifications

Shipping Information or Purchase Restrictions
  • Exclamation icon This item contains living or perishable material and ships via 2nd Day or Overnight delivery.
What’s Included:
  • E. coli Culture 
  • 1 pGREEN Plasmid Solution, 200 µL 
  • 1 Ampicillin, 4 mL 
  • 1 Sterile LB Agar, 400 mL
  • 20 Sterile Petri Dishes
  • 4 50 mM Calcium Chloride, 3 mL
  • 4 LB Broth, 3 mL
  • 8 Transformation Tubes, 15 mL
  • 24 Sterile Transfer Pipets, 1 mL
  • 1 Glass Beads, 1 oz
  • 15 Sterile Transfer Loops
  • 1 Wire Inoculating Loop
  • 1 Teacher's Manual and Reproducible Student Guide
  • expand to see full list
Needed But Not Included:
  • 1 Boiling Water Bath or Microwave (for melting agar)
  • 4 Beakers, 600 mL
  • 1 Container (for glass beads)
  • 4 Culture Tube Racks
  • 4 Wax Markers
  • 1 Masking Tape
  • 1 Bunsen Burner
  • 1 Water Bath, 42°C
  • 1 Autoclavable Disposable Bag
  •  Ice
  •  Parafilm® or Plastic Wrap
  •  Bleach Solution, 10%
  •  Incubator (optional)
  • expand to see full list
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