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800.334.5551

Colony Transformation 4-Station Kit (with perishables)

$82.90
(in stock)

Description

  • No liquid culturing
  • Minimal teacher preparation
Designed to match traditional AP® Biology Lab 6. This kit demonstrates the induced uptake and expression of foreign DNA by a living cell. Students observe the phenotypic effect of adding new DNA sequences to living bacteria. E. coli cells that take up an antibiotic-resistance gene gain the ability to grow in the presence of ampicillin. Requires preparation 2 days prior to lab.

The 4-Station Kit includes enough materials for 4 teams of students. Includes perishable materials when kit is shipped.

AP® is a trademark registered and/or owned by the College Board, which was not involved in the production of, and does not endorse, this product.

Specifications

Shipping Information or Purchase Restrictions
  • Exclamation icon This item contains living or perishable material and ships via 2nd Day or Overnight delivery.
What’s Included:
  • E. coli Culture 
  • 1 pAMP Plasmid Solution, 200 µL 
  • 1 Ampicillin, 4 mL 
  • 1 Sterile LB Agar, 400 mL
  • 20 Sterile Petri Dishes
  • 4 50 mM Calcium Chloride, 3 mL
  • 4 LB Broth, 3 mL
  • 8 Transformation Tubes, 15 mL
  • 24 Sterile Transfer Pipets, 1 mL
  • 1 Glass Beads, 1 oz
  • 15 Sterile Transfer Loops
  • 1 Wire Inoculating Loop
  • 1 Teacher's Manual and Reproducible Student Guide
  • expand to see full list
Needed But Not Included:
  • 1 Boiling Water Bath or Microwave (for melting agar)
  • 4 Beakers, 600 mL
  • 1 Container (for glass beads)
  • 4 Culture Tube Racks
  • 4 Wax Markers
  • 1 Masking Tape
  • 1 Bunsen Burner
  • 1 Water Bath, 42°C
  • 1 Autoclavable Disposable Bag
  •  Ice
  •  Parafilm® or Plastic Wrap
  •  Bleach Solution, 10%
  •  Incubator (optional)
  • expand to see full list
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