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This kit introduces bacterial transformation by demonstrating the induced uptake and expression of foreign DNA by a living cell. Students observe the phenotypic effect of adding new DNA sequences to living bacteria. E. coli cells that take up an antibiotic-resistance gene gain the ability to grow in the presence of ampicillin. Materials are sufficient for 4 student lab stations or groups; kit includes the perishable materials when shipped.
Product Details
IntermediateEasy to perform; requires some background knowledge.
How can genetic engineering be used for bacteria to gain resistance to an antibiotic?
This kit introduces bacterial transformation by demonstrating the induced uptake and expression of foreign DNA by a living cell. Students observe the phenotypic effect of adding new DNA sequences to living bacteria. E. coli cells that take up an antibiotic-resistance gene gain the ability to grow in the presence of ampicillin.
Product Features
Note: Kit includes the perishable materials when shipped.
Time Requirement
Teacher prep, prepare LB agar plates and streak start plates. Performing the lab, approximately one to two 60-minute class periods. Post-lab results, approximately one 60-minute class period.
Digital Resources
Includes 1-year access to digital resources that support instruction. Digital resources may include a teacher manual and student guide, pre-lab activities and setup videos, simulations, and post-lab analysis and assessments.
Connection to the Next Generation Science Standards*
Science and Engineering Practices
Performance Expectation(s)
Disciplinary Core Ideas
Crosscutting Concepts
Learning Objectives
Prerequisite Knowledge and Skills
Students should have a basic knowledge of cell structure and function, cell wall and membrane structure and function, and a working knowledge of protein synthesis.
AP® is a trademark registered and/or owned by the College Board®, which was not involved in the production of, and does not endorse, this product.
*Next Generation Science Standards® is a registered trademark of WestEd. Neither WestEd nor the lead states and partners that developed the Next Generation Science Standards were involved in the production of this product, and do not endorse it.