Add to List

This kit introduces bacterial transformation by demonstrating the induced uptake and expression of foreign DNA by a living cell. Students observe the phenotypic effect of adding new DNA sequences to living bacteria. E. coli cells that take up an antibiotic-resistance gene gain the ability to grow in the presence of ampicillin.

Intermediate—Easy to perform; requires some background knowledge.
This kit introduces bacterial transformation by demonstrating the induced uptake and expression of foreign DNA by a living cell. Students observe the phenotypic effect of adding new DNA sequences to living bacteria. E. coli cells that take up an antibiotic-resistance gene gain the ability to grow in the presence of ampicillin.

• Designed to match traditional AP® Biology Lab 6
• pAMP plasmid (item #211429) contains the gene for ampicillin resistance
• Included paper model activity simulates creation of recombinant plasmid with restriction enzymes
• Complete visual lab procedure complements the written protocol
• Resource section connects the kit activities to authentic lab techniques, real-world careers, historical perspectives, and more
• Kit content and the skills used by students during the investigations support obtainment of BCSI biotechnology micro-credentials
• Kit provides an NGSS phenomena activity and aligns to science and engineering practices and crosscutting concepts

Note: Order the kit with the perishable materials included or with a voucher to request perishables later at your convenience. Contact Carolina or return the voucher to request delivery of perishables.

AP® is a trademark registered and/or owned by the College Board®, which was not involved in the production of, and does not endorse, this product.