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Illuminate students' understanding of bacterial transformation and evolution by challenging them to genetically modify bacteria to glow in the dark. The genes involved in the production of light (also known as the lux genes) have been removed from V. fischeri and placed in a plasmid, pVIB. Bacterial transformation is then used to transfer the pVIB plasmid into E. coli. After E. coli takes up the plasmid, the bacteria glow in the dark.

Intermediate—Easy to perform; requires some background knowledge.

Illuminate your students' understanding of bacterial transformation and evolution by challenging them to genetically modify bacteria to glow in the dark! With this kit, the group of genes involved in the production of light (also known as the lux genes) has been removed from V. fischeri and placed in a plasmid, pVIB. Bacterial transformation is then used to transfer the pVIB plasmid into E. coli. After E. coli takes up the plasmid, the bacteria glow in the dark.

• Introduces the topic of transformation as the group of genes involved in production of light (lux genes) is transferred from V. fischeri to E. coli bacteria through their uptake of recombinant plasmid. Note: Bacteria transformed with the pVIB plasmid must be grown at 30° C or less for the glowing to be seen.
• pVIB plasmid (item #211447) contains genes for both ampicillin resistance and luciferase.
• Included paper model activity simulates creation of recombinant plasmid with restriction enzymes.
• Complete visual lab procedure complements the written protocol.
• Resource section connects the kit activities to authentic lab techniques, real-world careers, historical perspectives, and more.
• Kit content and the skills used by students during the investigations support obtainment of BCSI biotechnology micro-credentials.
• Kit provides an NGSS phenomena activity and aligns to science and engineering practices and crosscutting concepts.

Note: Order the kit with the perishable materials included or with a voucher to request perishables later at your convenience. Contact Carolina or return the voucher to request delivery of perishables.