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  • Glow-in-the-Dark Transformation Teacher Demonstration Kit (with perishables)

Glow-in-the-Dark Transformation Teacher Demonstration Kit (with perishables)

Item # 211086P With Digital This kit includes 1-year access to digital resources including videos, digital teacher’s manuals, printable student guides, interactive lessons, editable assessment questions, and more.
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Bacteria that produce light are very common in the ocean. The group of genes involved in the production of light (also known as the lux genes) has been removed from V. fischeri and placed in a plasmid, pVIB. Bacterial transformation is used to transfer the pVIB plasmid into E. coli. After E. coli takes up the plasmid, it glows in the dark. The transformed colonies are also ampicillin resistant. Perishable materials included.
Price

$61.15

In stock and available to ship.
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Overview

Intermediate - Easy to perform; requires some background knowledge.

Bacteria that produce light are very common in the ocean. The group of genes involved in the production of light (also known as the lux genes) has been removed from V. fischeri and placed in a plasmid, pVIB. Bacterial transformation is used to transfer the pVIB plasmid into E. coli. After E. coli takes up the plasmid, it glows in the dark. The transformed colonies are also ampicillin resistant. Kit includes perishable materials, but the kit and the perishable components will ship separately and may arrive on different days. Note: Bacteria transformed with the pVIB plasmid must be grown at 30° C or less for the glowing to be seen.
  • This item contains living or perishable material and ships via 2nd Day or Overnight delivery to arrive on a date you specify during Checkout. To ensure freshness during shipping, a Living Materials Fee may apply to orders containing these items.

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What's Included

This item ships with living or perishable materials (all items). Please select a delivery date during Checkout.

Components Qty Included?
E. coli Culture  1 Included
pVIB Plasmid Solution, 200 µL  1 Included
LB Agar Plates, Pre-poured  3 Included
LB Agar Plates with Ampicillin, Pre-poured  2 Included
50 mM Calcium Chloride, 3 mL 1 Included
LB Broth, 3 mL 1 Included
Transformation Tubes, 15 mL 2 Included
Sterile Transfer Pipets, 1 mL 7 Included
Glass Beads, 1 oz 1 Included
Sterile Transfer Loops 4 Included
Wire Inoculating Loop 1 Included
Teacher's Manual and Reproducible Student Guide 1 Included
Beaker, 600 mL 1 Needed, Not included
Container (for glass beads) 1 Needed, Not included
Culture Tube Rack 1 Needed, Not included
Wax Marker 1 Needed, Not included
Bunsen Burner 1 Needed, Not included
Water Bath, 42°C 1 Needed, Not included
Autoclavable Disposable Bag 1 Needed, Not included
Ice Needed, Not included
Bleach Solution, 10% Needed, Not included
Incubator (optional) Needed, Not included

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Carolina Biological and the Cold Spring Harbor Laboratory's DNA Learning Center (DNALC) have partnered together since 1986 to make molecular genetics and biotechnology more accessible to students globally. Focused on creating innovative classroom activities that give students the opportunity to go hands-on with DNA and explore the core principles of genetics. Together we have constantly worked to increase the accessibility of many key scientific breakthroughs for classrooms globally, including such techniques as PCR, DNA Sequencing, and RNAi.

Bringing a Carolina and DNALC kit into your classroom introduces your students to the experience of scientific research on its highest level, in some cases Nobel Prize winning research, with ease and simplicity. Each kit features in-depth background information, classroom tested protocols, pre-packaged materials, and the knowledgeable technical support of the Carolina Biological team. Our wide selection of kits in this series offers educators' unmatched opportunities to bring biotechnology or genetics into their classroom, with a best in class product.

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