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800.334.5551

Glow-in-the-Dark Transformation Teacher Demonstration Kit (with perishables)

$63.00
(in stock)

Description

Intermediate - Easy to perform; requires some background knowledge.

Bacteria that produce light are very common in the ocean. The group of genes involved in the production of light (also known as the lux genes) has been removed from V. fischeri and placed in a plasmid, pVIB. Bacterial transformation is used to transfer the pVIB plasmid into E. coli. After E. coli takes up the plasmid, it glows in the dark. The transformed colonies are also ampicillin resistant. Kit includes perishable materials, but the kit and the perishable components will ship separately and may arrive on different days. Note: Bacteria transformed with the pVIB plasmid must be grown at 30° C or less for the glowing to be seen.

Specifications

Shipping Information or Purchase Restrictions
  • Exclamation icon This item contains living or perishable material and ships via 2nd Day or Overnight delivery.
What’s Included:
  • E. coli Culture 
  • 1 pVIB Plasmid Solution, 200 µL 
  • 3 LB Agar Plates, Pre-poured 
  • 2 LB Agar Plates with Ampicillin, Pre-poured 
  • 1 50 mM Calcium Chloride, 3 mL
  • 1 LB Broth, 3 mL
  • 2 Transformation Tubes, 15 mL
  • 7 Sterile Transfer Pipets, 1 mL
  • 1 Glass Beads, 1 oz
  • 4 Sterile Transfer Loops
  • 1 Wire Inoculating Loop
  • 1 Teacher's Manual and Reproducible Student Guide
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Needed But Not Included:
  • 1 Beaker, 600 mL
  • 1 Container (for glass beads)
  • 1 Culture Tube Rack
  • 1 Wax Marker
  • 1 Bunsen Burner
  • 1 Water Bath, 42°C
  • 1 Autoclavable Disposable Bag
  •  Ice
  •  Bleach Solution, 10%
  •  Incubator (optional)
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