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Glow-in-the-Dark Transformation Teacher Demonstration Kit (with voucher)

$63.00
(in stock)

Description

Intermediate - Easy to perform; requires some background knowledge.

Bacteria that produce light are very common in the ocean. The group of genes involved in the production of light (also known as the lux genes) has been removed from V. fischeri and placed in a plasmid, pVIB. Bacterial transformation is used to transfer the pVIB plasmid into E. coli. After E. coli takes up the plasmid, it glows in the dark. The transformed colonies are also ampicillin resistant. Kit includes a voucher to request living materials later at your convenience. Contact us or return the voucher to request delivery of living materials. Note: Bacteria transformed with the pVIB plasmid must be grown at 30° C or less for the glowing to be seen.

Specifications

What’s Included:
  • E. coli Culture (voucher item)
  • 1 pVIB Plasmid Solution, 200 µL (voucher item)
  • 3 LB Agar Plates, Pre-poured (voucher item)
  • 2 LB Agar Plates with Ampicillin, Pre-poured (voucher item)
  • 1 50 mM Calcium Chloride, 3 mL
  • 1 LB Broth, 3 mL
  • 2 Transformation Tubes, 15 mL
  • 7 Sterile Transfer Pipets, 1 mL
  • 1 Glass Beads, 1 oz
  • 4 Sterile Transfer Loops
  • 1 Wire Inoculating Loop
  • 1 Teacher's Manual and Reproducible Student Guide
Needed But Not Included:
  • 1 Beaker, 600 mL
  • 1 Container (for glass beads)
  • 1 Culture Tube Rack
  • 1 Wax Marker
  • 1 Bunsen Burner
  • 1 Water Bath, 42°C
  • 1 Autoclavable Disposable Bag
  •  Ice
  •  Bleach Solution, 10%
  •  Incubator (optional)
Return Policy:

If for any reason you are not satisfied with this item, it is eligible for a return, exchange, refund, or credit up to 180 days from date of purchase. Restrictions may apply. Returns & Exchanges Policy.

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