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Inducing RNAi by Feeding Kit

4 Items With Digital This kit includes 1-year access to digital resources including videos, digital teacher’s manuals, printable student guides, interactive lessons, editable assessment questions, and more.
$5.50 - $195.85 View Details

Advanced – For experienced high school and college classes; requires some technical skill.
Demonstrate to your students the power of silencing a single gene. RNA interference (RNAi) is a technique that allows you to silence the expression of a chosen gene by specifically degrading its mRNA. In this kit, students use this Nobel Prize-winning technique to silence 2 different genes in the nonparasitic roundworm C. elegans, a model organism used for studying many aspects of biology.

 
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Overview

Advanced – For experienced high school and college classes; requires some technical skill.

  • Demonstrate to your students the power of silencing a single gene.
  • Teach about a powerful method for determining gene function.
  • Introduce your students to a model organism used for studying many aspects of biology, including development and gene function.
  • Engage in bioinformatics exercises exploring protein function and C. elegans and human gene relatedness.
In this lab, wild-type worms are fed lab strains of E. coli that express double-stranded RNA (dsRNA) corresponding to either of 2 target genes: BLIstered cuticle (bli-1) or DumPY (dpy-11). The ingestion of the dsRNA initiates the destruction of mRNA expressed from the target genes. Students then examine the phenotypes induced by silencing these genes. Silencing bli-1 leads to the formation of clear blisters in a worm's outer cuticle; silencing dpy-11 leads to a worm developing a short, fat body. In accompanying bioinformatics exercises, students explore the function of the protein encoded by the silenced gene and the relatedness of worm and human genes.

Note: Order the kit with the perishable materials included (item #211391P) or with a prepaid coupon to request perishables later at your convenience (item #211391). Because of the time-sensitive preparation and limited shelf life of the kit's perishable materials, please order a minimum of 2 weeks prior to the desired ship date. Plan on using the materials within a week of receiving them. Perishables ship only on Mondays, Tuesdays, and Wednesdays via standard overnight delivery.

 

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