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  • pBLU® Colony Transformation 8-Station Kit (with perishables)

pBLU® Colony Transformation 8-Station Kit (with perishables)

Item # 211146P With Digital This kit includes 1-year access to digital resources including videos, digital teacher’s manuals, printable student guides, interactive lessons, editable assessment questions, and more.
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Designed to match traditional AP® Biology Lab 6. Students use specially developed pBLU® plasmid in a colony transformation procedure to observe the phenotypic effect of inserting new genes into living bacteria. The 8-Station Kit includes enough materials for 8 teams of students. Includes perishable materials when kit is shipped.

Price

$126.75

In stock and available to ship.
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Overview

Thanks to the dramatic color change and ampicillin, students clearly see the results of introducing new genes into live bacteria

  • Great for teaching the molecular basis of inheritance
  • Simple, straightforward procedure
  • Minimizes teacher prep time
Designed to match traditional AP® Biology Lab 6. Students use specially developed pBLU® plasmid in a colony transformation procedure to observe the phenotypic effect of inserting new genes into living bacteria. Transformants appear as white colonies on the ampicillin medium and as dramatically blue colonies on the ampicillin/X-gal medium. The 8-Station Kit includes enough materials for 8 teams of students. Includes perishable materials when kit is shipped.

AP® is a trademark registered and/or owned by the College Board, which was not involved in the production of, and does not endorse, this product.

  • This item contains living or perishable material and ships via 2nd Day or Overnight delivery to arrive on a date you specify during Checkout. To ensure freshness during shipping, a Living Materials Fee may apply to orders containing these items.

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What's Included

This item ships with living or perishable materials (all items). Please select a delivery date during Checkout.

Components Qty Included?
E. coli Culture  1 Included
pBLU® Plasmid Solution, 200 µL  1 Included
Ampicillin, 4 mL  1 Included
Ampicillin/X-gal Solution, 2% in DMF, 5.4 mL  1 Included
Sterile LB Agar, 400 mL 3 Included
Sterile Petri Dishes 50 Included
50 mM Calcium Chloride, 3 mL 8 Included
LB Broth, 3 mL 8 Included
Transformation Tubes, 15 mL 16 Included
Sterile Transfer Pipets, 1 mL 48 Included
Glass Beads, 1 oz 2 Included
Sterile Transfer Loops 28 Included
Wire Inoculating Loop 1 Included
Teacher's Manual and Reproducible Student Guide 1 Included
Boiling Water Bath or Microwave (for melting agar) 1 Needed, Not included
Beakers, 600 mL 8 Needed, Not included
Container (for glass beads) 1 Needed, Not included
Culture Tube Racks 8 Needed, Not included
Wax Markers 8 Needed, Not included
Masking Tape 1 Needed, Not included
Bunsen Burner 1 Needed, Not included
Water Bath, 42°C 1 Needed, Not included
Autoclavable Disposable Bag 1 Needed, Not included
Ice Needed, Not included
Parafilm® or Plastic Wrap Needed, Not included
Bleach Solution, 10% Needed, Not included
Incubator (optional) Needed, Not included

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