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pBLU® Colony Transformation 8-Station Kit (with perishables)

$130.55
Product Highlights
  • Easily visualize transformation with the pBLU® plasmid
  • Includes perishable materials when kit is shipped
  • Refills can be purchased separately
(in stock)

Description

Product Details
Intermediate—Easy to perform; requires some background knowledge.

This impactful transformation kit is designed to make observing the phenotypic effects of transformation easier than ever. Thanks to the specially developed pBLU® plasmid, transformants appear as white colonies on an ampicillin medium and as dramatic blue colonies on an ampicillin/X-gal medium. Kit includes perishable materials.

Product Features

  • Great for teaching the molecular basis of inheritance
  • Simple, straightforward procedure
  • Designed to match the traditional AP® Biology Lab 6
  • Designed for 8 student groups/lab stations
  • pBLU® plasmid contains gene for ampicillin resistance

AP® is a trademark registered and/or owned by the College Board, which was not involved in the production of, and does not endorse, this product.

Time Requirement
Teacher prep, prepare LB agar plates and streak start plates. Performing lab, approximately one to two 60-minute class periods. Post lab results, approximately one 60-minute class period.

Digital Resources
Includes 1-year access to digital resources that include a teacher’s manual, student guide, fill-in answer sheets, whiteboard resources, videos, interactive digital prelab activity, and more.

Performance Expectation(s)
HS-LS1-1
HS-LS3-1

Crosscutting Concepts
Cause and Effect

Disciplinary Core Ideas
HS LS1.A: Structure and Function

Science and Engineering Practices
Constructing Explanations and Designing Solutions

Learning Objectives

  • Explain how one organism can be genetically engineered to produce a protein from a different organism.
  • Draw connections between the techniques performed in this laboratory and the development of current critical pharmaceuticals.
  • Explain the use and importance of selective marker genes in a bacterial transformation.

Prerequisite Knowledge and Skills
Students should have a basic knowledge of cell structure and function, cell wall and membrane structure and function, and a working knowledge of protein synthesis.

Specifications

Shipping Information or Purchase Restrictions
  • Exclamation icon This item contains living or perishable material and ships via 2nd Day or Overnight delivery.
What’s Included:
  • E. coli Culture 
  • 1 pBLU® Plasmid Solution, 200 µL 
  • 1 Ampicillin, 4 mL 
  • 1 Ampicillin/X-gal Solution, 2% in DMF, 5.4 mL 
  • 3 Sterile LB Agar, 400 mL
  • 50 Sterile Petri Dishes
  • 8 50 mM Calcium Chloride, 3 mL
  • 8 LB Broth, 3 mL
  • 16 Transformation Tubes, 15 mL
  • 48 Sterile Transfer Pipets, 1 mL
  • 2 Glass Beads, 1 oz
  • 28 Sterile Transfer Loops
  • 1 Wire Inoculating Loop
  • 1 Teacher's Manual and Reproducible Student Guide
  • expand to see full list
Needed But Not Included:
  • 1 Boiling Water Bath or Microwave (for melting agar)
  • 8 Beakers, 600 mL
  • 1 Container (for glass beads)
  • 8 Culture Tube Racks
  • 8 Wax Markers
  • 1 Masking Tape
  • 1 Bunsen Burner
  • 1 Water Bath, 42°C
  • 1 Autoclavable Disposable Bag
  •  Ice
  •  Parafilm® or Plastic Wrap
  •  Bleach Solution, 10%
  •  Incubator (optional)
  • expand to see full list
Return Policy:

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