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pBLU® Colony Transformation Teacher Demonstration Kit (with perishables)

$75.35
(in stock)

Description

Thanks to the dramatic color change and ampicillin, students clearly see the results of introducing new genes into live bacteria

  • Great for teaching the molecular basis of inheritance
  • Simple, straightforward procedure
  • Minimizes teacher prep time
Designed to match traditional AP® Biology Lab 6. Students use specially developed pBLU® plasmid in a colony transformation procedure to observe the phenotypic effect of inserting new genes into living bacteria. Transformants appear as white colonies on the ampicillin medium and as dramatically blue colonies on the ampicillin/X-gal medium. The Teacher Demonstration Kit includes enough materials for 1 demonstration. Includes perishable materials when kit is shipped.

AP® is a trademark registered and/or owned by the College Board, which was not involved in the production of, and does not endorse, this product.

Specifications

Shipping Information or Purchase Restrictions
  • Exclamation icon This item contains living or perishable material and ships via 2nd Day or Overnight delivery.
What’s Included:
  • E. coli Culture 
  • 1 pBLU® Plasmid Solution, 200 µL 
  • 3 LB Agar Plates, Pre-poured 
  • 2 LB Agar Plates with Ampicillin, Pre-poured 
  • 2 LB Agar Plates with X-gal Solution, Pre-poured 
  • 1 50 mM Calcium Chloride, 3 mL
  • 1 LB Broth, 3 mL
  • 2 Transformation Tubes, 15 mL
  • 7 Sterile Transfer Pipets, 1 mL
  • 1 Glass Beads, 1 oz
  • 4 Sterile Transfer Loops
  • 1 Wire Inoculating Loop
  • 1 Teacher's Manual and Reproducible Student Guide
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Needed But Not Included:
  • 1 Beaker, 600 mL
  • 1 Container (for glass beads)
  • 1 Culture Tube Rack
  • 1 Wax Marker
  • 1 Bunsen Burner
  • 1 Water Bath, 42°C
  • 1 Autoclavable Disposable Bag
  •  Ice
  •  Bleach Solution, 10%
  •  Incubator (optional)
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