Add to List

Teach your students how to purify a green fluorescent jellyfish protein produced in E. coli!

Bringing genetic engineering and protein purification into your classroom has never been simpler. Module 2 is designed to allow students to purify GFP from the E. coli cells they successfully engineered in a previous transformation experiment. This lab activity requires prior completion of Module 1, Green Gene Colony Transformation Kit (item #211080, #211080P, #211082, or #211082P).

Intermediate - Requires some background knowledge.

This kit module is great for teaching:

• The molecular basis of heredity
• The relationship between genes and proteins
• How E. coli is used to engineer proteins useful in medicine and manufacturing
• Three techniques - transformation, protein purification, and protein gel electrophoresis - commonly used in biological research and its areas of application

This lab activity is part of a series set up in 3 modules that can be purchased separately and used in conjunction with one another. Successful completion of Module 2 is dependent on prior results from Module 1, Green Gene Colony Transformation Kit (item #211080, #211080P, #211082, or #211082P).

In this activity students take their successfully transformed bacteria and use HIC resin to conduct a batch purification technique. This purification process doesn't require the time or expense typically associated with column-based purification protocols; instead, it produces quick results in 1 class period (40 minutes).

Note: Kit ships with the perishable materials included.