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800.334.5551

Transduction of an Antibiotic-Resistance Gene Kit (with perishables)

$193.20
(in stock)

Description

Intermediate—Easy to perform; requires some background knowledge.

In this easy exercise, students observe a natural method of gene transfer. The harmless bacterial virus T4 transmits an ampicillin-resistance gene to E. coli. Students also observe the killing of E. coli by the bacteriophage. For more advanced students, a discussion of nonsense mutations and nonsense suppressing mutations is included with the teacher background information. Kit comes with perishable materials. Kit is designed for 16 to 32 students working in groups of 2 or 4 (8 stations).

Specifications

Shipping Information or Purchase Restrictions
  • Exclamation icon This item contains living or perishable material and ships via 2nd Day or Overnight delivery.
What’s Included:
  • E. coli BE Slant Culture 
  • E. coli CR63 Slant Culture 
  • 1 Transducing Lysate, 300 µL 
  • 2 Ampicillin, 10 mg/mL, 4 mL 
  • 2 Glass Beads, 1 oz
  • 4 Luria Broth Agar, 400 mL
  • 3 Luria Broth, 50 mL
  • 4 Sterile Pipets, 1 mL
  • 2 Sterile Pipets, 10 mL
  • 100 Sterile Culture Tubes
  • 4 Inoculating Loops
  • 65 Sterile Petri Plates
  • 24 Sterile Transfer Pipets
  • 1 Digital Resource Instruction Card
  • 1 Teacher’s Manual and Reproducible Student Guide
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Needed But Not Included:
  •  Micropipets (10 µL and 50 µL)
  •  Sterile Tips for Micropipets
  •  Sterile Microcentrifuge Tubes
  •  Pipet Bulb or Automatic Pipet
  • 8 Large Waste Beakers
  • 8 Large Glass Bead Collection Beakers
  • 1 Boiling Water Bath (or microwave)
  •  Test Tube Racks
  •  Permanent Marker
  •  Bunsen Burner
  •  Incubator, 37° C (optional)
  •  Water Bath, 55° C (optional)
  • expand to see full list
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