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Description
In this easy exercise, students observe a natural method of gene transfer. The harmless bacterial virus T4 transmits an ampicillin-resistance gene to E. coli. Students also observe the killing of E. coli by the bacteriophage. For more advanced students, a discussion of nonsense mutations and nonsense suppressing mutations is included with the teacher background information.
Intermediate—Easy to perform; requires some background knowledge.
In this easy exercise, students observe a natural method of gene transfer. The harmless bacterial virus T4 transmits an ampicillin-resistance gene to E. coli. Students also observe the killing of E. coli by the bacteriophage.
For more advanced students, a discussion of nonsense mutations and nonsense suppressing mutations is included with the teacher background information. Kit is designed for 16 to 32 students working in groups of 2 or 4 (8 stations).
Note: Order the kit with the perishable materials included or with a voucher to request perishables later at your convenience. Contact us ore return the voucher to request delivery of perishable materials.
