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Description
This kit explores the molecular basis of the inherited ability to taste the bitter chemical phenylthiocarbamide (PTC). Students determine their ability to taste PTC using taste paper. They then use safe saline mouthwash and Chelex® extraction to obtain a sample of their own DNA and amplify a 221-nucleotide region of the PTC taste receptor gene.
Advanced - For experienced high school and college classes; requires some basic technical skill.
The PTC Taster: Using SNP to Predict Bitter Tasting Ability Kit explores the molecular basis of an inherited ability by connecting students' own ability to taste the bitter chemical phenylthiocarbamide (PTC) to molecular genetics. Each student scores their own genotype, predicts their tasting ability, then tastes PTC paper. Class results show how well PTC tasting conforms to classical Mendelian inheritance. Students then use safe saline mouthwash and Chelex® extraction to obtain a sample of their own DNA and amplify a 221-nucleotide region of the PTC taste receptor gene to demonstrate if they do or do not have the TAS2R38 gene for tasting PTC.
Kit provides materials for 25 PCR reactions and restriction digests. Note: Order the kit with the perishable materials included or with a voucher to request perishables later at your convenience. Contact us or return the voucher to request delivery of perishable materials.
