Using a Single Nucleotide Polymorphism (SNP) to Predict Bitter Tasting Ability Kit | Carolina.com

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Using a Single Nucleotide Polymorphism (SNP) to Predict Bitter Tasting Ability Kit

9 Items
Using a Single Nucleotide Polymorphism (SNP) to Predict Bitter Tasting Ability Kit is rated 3.9 out of 5 by 8.
  • y_2018, m_12, d_10, h_15
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$33.50 - $255.00 View Details

This kit explores the molecular basis of the inherited ability to taste the bitter chemical phenylthiocarbamide (PTC). Students determine their ability to taste PTC using taste paper. They then use safe saline mouthwash and Chelex® extraction to obtain a sample of their own DNA and amplify a 221-nucleotide region of the PTC taste receptor gene.

 
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This kit explores the molecular basis of the inherited ability to taste the bitter chemical phenylthiocarbamide (PTC). Students determine their ability to taste PTC using taste paper. They then use safe saline mouthwash and Chelex® extraction to obtain a sample of their own DNA and amplify a 221-nucleotide region of the PTC taste receptor gene. The 2 alleles differ by a single nucleotide, so restriction digestion of the amplified product followed by gel electrophoresis effectively differentiates the 2 alleles. One allele strongly correlates to the ability to taste PTC. After scoring their SNP genotypes, students determine how well the SNP genotypes actually correlate to tasting.

Each kit includes materials for 25 PCR reactions and restriction digests, and a PTC CD-ROM. CD-ROM includes animations and additional resources.

** Kit and perishable components will ship separately and may arrive on different days.

 
 
 
 
Rated 1 out of 5 by from Complicated Procedure, no results, etc Our gels at the end of this lab showed no DNA bands from the students. That happens occasionally with this lab. It would be on my students but it is the first time that NO students got results. Also, the instructions for this lab (especially the student version) are much more complicated than other companies instructions. I spent alot more time in prelab each day going over the steps and what to do. Finally, when I received the product it did not come with agarose, buffer, or extra 0.2 mL microcentrifuge tubes. All of these are needed in the lab. I called Carolina and they told me that the kit does not come with them. The picture that Carolina shows on the website shows all of these things in it. Luckily I had all of these already in my lab but if not I would have had to make another order and wait for these items to come in.
Date published: 2018-11-07
Rated 2 out of 5 by from DNA bands too faint to see well The kit is very straight forward to use and the protocol is well written with easy-to-follow instructions. It is very engaging since students get to discover something about themselves. The main problem we had was that the bands were so faint it was impossible to draw any conclusions. We added more cycles as suggested in the protocol, but bands were still too faint. We used Carolina Blue. The photos in the booklet are from gels stained with ethidium bromide and those results look good. We cannot use EtBr, so sadly we will not be able to adopt this exercise.
Date published: 2017-07-06
Rated 5 out of 5 by from Worked great for us! The kit was very straight forward and easy to use. Results were clean and simple to interpret.
Date published: 2014-11-21
Rated 5 out of 5 by from Great product to use! I have been using this lab kit for several years now and each year it is super easy to follow and the kids love it. It's great to show how just a SNP can cause such a huge difference in a taste. The set up is easy and the directions are straight forward. I do find that some students have trouble understanding the genetics behind it, but the lab provides excellent material for you to go over with the students to help them see what is going on. The one thing I would love to see changed is to add the enzyme HaeIII automatically to the refill kit. Other than that, this is a great product.
Date published: 2014-11-21
Rated 4 out of 5 by from Faint results, but still good Just finished this lab. Great results, but student DNA is very faint on the gel. Suggest adding more cycles to PCR. Overall, good lab!
Date published: 2014-11-19
Rated 5 out of 5 by from Near fail-proof I use this kit early in the semester in my 200-level genetics course. Students are engaged because it is an experiment on themselves and they get to learn something new about themselves. The kit works great. I rarely have a student fail to get a readable result. It is a little "below" the level I like for a 200-level undergraduate course but I address this by asking them manipulate the data in ways that are not specifically included in the kit. We talk about incomplete dominance (this locus does show some incomplete dominance). We compare our class genotypic and phenotypic frequencies to the overall population and ask if our class is in Hardy-Weinberg equilibrium. In brief: pros: easy and effective, almost always produces results cons: expensive, at a lower level than I would like (but easy to adjust up a few levels)
Date published: 2014-11-19
Rated 4 out of 5 by from PCR for Bitter tasting Overall good results. However the bioinformatics section needs to be updated. The current protocol is not helpful.
Date published: 2014-04-11
Rated 5 out of 5 by from Worked Great! I have seen bad reviews of this product from others. It won't work right if you use a 1.2% agarose gel. It isn't enough to separate the bands. I accidentally missed this fact the first time I did it and the lab resulted in one band for all students. When I did it this year and changed it to 2.0% agarose (which the directions say to use) it worked great! Even better...The genotype that the lab showed for the students matched 95% of my students based on their ability to taste. They learned a lot and enjoyed this lab. Definitely more fun than the ALU insertion as it gives some very interesting information.
Date published: 2012-01-06
  • y_2018, m_12, d_10, h_15
  • bvseo_bulk, prod_bvrr, vn_bulk_2.0.9
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_8
  • loc_en_US, sid_FAM_211376, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_carolina
  • bvseo_sdk, java_sdk, bvseo-4.0.0
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