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BioBuilder® Golden Bread PCR Kit (with perishables)

Item # 217008P Online Only This product is not available in our print catalog. Exclusive This product is exclusive to Carolina Biological Supply.

Advanced—For experienced high school and college classes; requires some technical skill.
This biology lab explores the engineering of a yeast that's genetically modified to produce ß-carotene. Use PCR to test the metabolic pathway of modified Saccharomyces cerevisiae as part of the engineering design-build-test pathway and investigate how to make it possible for users to bake vitamin A-enriched loaves of bread.

Price

$137.00

In stock and available to ship.

Advanced—For experienced high school and college classes; requires some technical skill.

  • Introduce bio-engineering concepts
  • Teach synthetic biology and microbiology techniques
  • Allow students to experience real-world connections

This biology lab explores the engineering of a yeast that's genetically modified to produce ß-carotene. In the body, ß-carotene is converted to vitamin A, which is crucial for vision, the immune system, and other biological functions. Use PCR to test the metabolic pathway of modified Saccharomyces cerevisiae as part of the engineering design-build-test pathway and investigate how to make it possible for users to bake vitamin A-enriched loaves of bread. Kit includes enough materials for 24 students working in groups of 4.

Students will perform:

  • Cell culture of the modified yeast on petri dishes
  • DNA extraction from yeast colonies
  • PCR to amplify genes from yeast
  • Electrophoresis to visualize results (electrophoresis materials are included in the item #217009 BioBuilder® Golden Bread PCR Electrophoresis Pack)

This lab may be performed on its own or in conjunction with the BioBuilder® Golden Bread Transformation Kit (items #217006 and #217006P). PCR could be used to test for gene content of different colored colonies before and after transformation. The transformation kit inserts a second copy of one of the enzymes needed in the biosynthetic pathway of ß-carotene, scientifically addressing the importance of genetic redundancy when engineering synthetic systems.

  • This item is only available from Carolina Biological Supply Company.
  • This item contains living or perishable material and ships via 2nd Day or Overnight delivery to arrive on a date you specify during Checkout. To ensure freshness during shipping, a Living Materials Fee may apply to orders containing these items.
  • This item is only available at Carolina.com.

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This item ships with living or perishable materials (all   items). Please select a delivery date during checkout.

Components Qty Included?
VitaYeast Culture  1 Included
Positive Control  1 Included
DNA Primer Mix  1 Included
Petri Dishes 25 Included
Inoculating Needles 30 Included
Microcentrifuge Tubes 30 Included
Ready-to-Go PCR Beads 30 Included
Melt & Pour YED Media, 350 mL 1 Included
X-Tract™ Buffer 1 Included
Sterile Water, 1 mL 1 Included
BioBuilder® Instruction Card 1 Included

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