Add to List

Bring bioinformatics to the classroom in an accessible manner. Students use "DNA barcodes"—short, unique DNA sequences—to learn about the biodiversity of plants, mammals, fish, or insects. Using PCR on specific regions of the rbcL or COI gene, students amplify their extracted DNA to create unique barcodes. These amplified sequences are then submitted for sequencing and can be analyzed using an online tool, DNA Subway. Each kit contains enough materials for 25 DNA extractions and PCR reactions.

Advanced—For experienced high school and college classes; requires some technical skill.

Explore evolution and taxonomy in molecular biology by bringing bioinformatics to the classroom in an accessible manner. Students take a new approach to taxonomy using "DNA barcodes"—short, unique DNA sequences—to learn about the biodiversity of plants, mammals, fish, or insects. Using polymerase chain reaction (PCR) on specific regions of the rbcL or COI gene, students amplify their extracted DNA to create unique barcodes. These amplified sequences (amplicons) are then submitted for sequencing and can be analyzed using a custom-designed online tool, DNA Subway. Designed, tested, and updated with the classroom in mind, the revised protocol saves time and delivers robust results.

• Sufficient materials for 25 DNA extractions and PCR reactions
• Unlock the barcodes of even more organisms by purchasing the Fungal Primer Set (item #211388, sold separately)
• Included paper model activity simulates cycles of polymerase chain reactions
• A complete visual lab procedure complements the written protocol
• Resource section connects the kit activities to authentic lab techniques, real-world careers, historical perspectives, and more
• Kit content and the skills used by students during the investigations support the obtaining of BCSI biotechnology micro-credentials
• Provides an NGSS phenomena activity and aligns to science and engineering practices and crosscutting concepts

Note: Order the kit with the perishable materials included or with a voucher to request perishables later at your convenience. Contact Carolina or return the voucher to request delivery of perishable materials.