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Identifying the Mutation in Non-Purple Stem Wisconsin Fast Plants® Amplification Kit with 0.2-mL Tubes (with perishables)

$190.55
(in stock)

Description

Advanced—For experienced high school and college classes; requires some technical skill.

Making a genotype-to-phenotype connection using Wisconsin Fast Plants®

This lab explores the connection between Mendelian genetics and molecular biology through the analysis of the mutation responsible for the non-purple stem trait in Wisconsin Fast Plants®. Students predict the phenotype and genotype for stem color in the F2 generation of a monohybrid cross and propose an explanation as to why the anl allele leads to a non-purple stem phenotype, whereas the ANL allele leads to the purple stem phenotype. Students finally plant F2 seeds on a monohybrid cross that will be used for phenotypic and molecular analysis. This lab also requires students to familiarize themselves with bioinformatics programs in which they practice analyzing sequence data of purple stem and non-purple stem alleles of the anthocyaninless genes. They then translate the sequences and discuss how the mutations found in non-purple stem alleles impact gene function. Kit equips 24 students working in groups of 4 and includes free 1-year access to digital resources that support 3-dimensional instruction for NGSS. Note: Kit includes perishable materials when shipped.

Specifications

Shipping Information or Purchase Restrictions
  • Exclamation icon Excepted Quantity - This product is/contains a DOT regulated hazardous material in an excepted quantity. This does not incur a hazardous materials fee. Orders shipping to HI and AK are subject to review and may incur additional shipping charges. This item contains living or perishable material and ships via 2nd Day or Overnight delivery.
What’s Included:
  • 1 Primer/Loading Dye Mix 
  • 1 W Primer/Loading Dye Mix 
  • 1 pBR322/BstN1 Marker 
  • 1 TE (Tris/EDTA) Buffer with RNase, 3 mL 
  • 30 Wisconsin Fast Plants® Seed, P1, Non-Purple Stem Hairless
  • 30 Wisconsin Fast Plants® Seed, P2, Purple Stem Hairy
  • 50 Wisconsin Fast Plants® Seed, F2, Non-Purple Stem Hairless x Purple Stem Hairy
  • 25 Petri Dishes
  • 100 Filter Paper, 9 cm
  • 1 Edwards Buffer, 20 mL
  • 15 Pellet Pestles
  • 25 PCR Beads in 0.2-mL PCR Tubes
  • 1 Isopropanol, 10 mL
  • 1 Carolina Science Online® Resources (1-year subscription)
  • 1 Teacher’s Manual with Reproducible Student Guide
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Needed But Not Included:
  •  Micropipets and Tips, 1 µL to 1,000 µL
  •  Microcentrifuge Tubes, 1.5 mL
  •  Microcentrifuge for 1.5-mL Tubes
  •  PCR Tubes, 0.2 mL or 0.5 mL
  •  Tube Adapters for Microcentrifuge, 0.2 mL or 0.5 mL
  •  Programmable Thermal Cycler
  •  Electrophoresis Chambers
  •  Electrophoresis Power Supplies
  •  Container with Cracked or Crushed Ice
  •  Water
  •  Camera (optional)
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