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Students collect the supernatant from ampicillin-resistant bacterial cultures and perform a simple colorimetric assay to detect the activity of beta-lactamase, the enzyme conferring ampicillin resistance. Contains perishable materials. Kit includes: E. coli slant, ampicillin-resistant E. coli slant, ampicillin, LB plate and LB/AMP plate, LB broth, penicillin G and phenol red, sterile culture tubes, microcentrifuge tubes, and instructions.
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