DNA Damage: Studying the Impact of UV Light 1-Station Kit (with perishables) | Carolina.com

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DNA Damage: Studying the Impact of UV Light 1-Station Kit (with perishables)

Item # 173609P Exclusive
DNA Damage: Studying the Impact of UV Light 1-Station Kit (with perishables) is rated 5.0 out of 5 by 2.
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Explaining the importance of DNA repair systems in your classroom doesn't always click with students. Help shine a light on this concept, literally, by having your students observe the impact of ultraviolet (UV) light on yeast strains. This kit uses 2 yeast strains, wild-type and a UV-sensitive mutant, with an inquiry-based approach to get your students talking about the importance of DNA repair systems and the impact of UV damage. Includes enough materials for 4 students.

Price

$30.00

In stock and available to ship.

After investigating the effect ultraviolet light has on different strains of yeast cells, students design an experiment to test the effectiveness of different ultraviolet preventatives.

PE: HS-LS3-2
Time Requirement: 3 45-minute Class Periods

SEP: Planning and Carrying out Investigations
CCC: Cause and Effect; Engaging in Argument from Evidence
DCI: LS3.B: Variation of Traits

A fresh inquiry update to our popular Sunscreen for Yeast - and People Too! Kit

  • Directly demonstrate the importance of DNA repair systems
  • Meaningful real-world connection for students
  • Learn how to best prevent/minimize exposure to ultraviolet (UV) light

Using a UV-sensitive mutant strain of the yeast Saccharomyces cerevisiae, students investigate the impact of UV light on living organisms. The S. cerevisiae mutant strain is extremely sensitive to UV light because it lacks an effective method for repairing damaged DNA. In the first activity, students determine the impact of UV light on 2 strains of yeast, wild-type and UV-sensitive. After understanding the true impact of UV light, students then have the opportunity to design their own inquiry investigation to determine how to best prevent UV damage to the UV-sensitive yeast strain. In the second activity, these investigations can have students testing various sunscreens, cloth samples, or myriad other options of their own choosing. This entire activity is accomplished in 3 to 4 class periods and will truly give you a great starting point for discussing DNA repair systems and the impact of UV light on the human body. This 1-Station Kit includes enough materials for 1 group of 4 students.

  • This item is only available from Carolina Biological Supply Company.
  • This item contains living or perishable material and ships via 2nd Day or Overnight delivery to arrive on a date you specify during Checkout. To ensure freshness during shipping, a Living Materials Fee may apply to orders containing these items.

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This item ships with living or perishable materials (all   items). Please select a delivery date during checkout.

Components Qty Included?
Wild-Type Yeast Culture  1 Included
UV-Sensitive Yeast Culture  1 Included
Sterile Toothpicks, Box 1 Included
Petri Dishes 20 Included
YED Media, 350 mL 1 Included
Culture Tubes, Sterile 3 Included
Transfer Pipets, 1 mL 5 Included
Sterile Distilled Water, 20 mL 1 Included
Glass Beads, 1 oz 1 Included
Teacher’s Manual with Reproducible Student Guide 1 Included
Aluminum Foil Needed, Not Included
Plastic Wrap Needed, Not Included
Waste Container Needed, Not Included
Ethanol or Bleach Needed, Not Included
UV Light Box (optional) 1 Optional, Not Included
Incubator (optional) Needed, Not Included
Test Tube Rack 1 Needed, Not Included
Permanent Marker 1 Needed, Not Included
Timers 1 Needed, Not Included
Various Types of Sunscreen (optional for inquiry) Needed, Not Included
Various Types of Fabric (optional for inquiry) Needed, Not Included
Rated 5 out of 5 by from Everything was perfect The kit arrived on time with everything intact. Project moving along well.
Date published: 2019-04-03
Rated 5 out of 5 by from Very complete kit The supplies and manual that come with this kit are very complete and impressive! This was purchased to do a High School Honors Biology and Science Fair project. I bought 2 kits so we had backup material and was glad I did so. I made some modifications to the instructions (beings a scientist myself I was capable to do so) and had to fill in some gaps. I have many years of laboratory experience working with agars, petri dishes and plating out live cultures. I would imagine this would be a tough process for a lay person. I am giving it 5 stars because it is a great kit, and seems to be meant for science teachers who I guess, should have some experience with these kinds of experiments. For a non-science person, it may be a struggle if you've never dealt with this before. The agar didn't melt that well. That was the biggest challenge to the case. I had to tweak my techniques alot, it didn't handle like many other agars I've used in the past (DMEM, etc) and is more of a gel that hardens quickly. I still give the kit 5 stars. I had to do some pilot studies to make sure I could get the experiment to work in my hands, and that's why I'm glad I bought 2 kits. But science is always like that and one shouldn't think this is just a science kit in a box and you follow instructions and it works. I spent weeks and went through 2 kits entirely before we figured out everything appropriately. Having experience in the lab however, this is just biology and science and I still give the kit 5 stars. Doing this experiment in the winter was challenging. So I used a UV lamp to kill the UV sensitive yeast, and the instructions don't really have that as a chart and every lamp is different. So once again, I had to do some pilot studies to figure out when my lamp killed the yeast, and then we did the final experiments. For $30, this is an amazing deal. And my high school daughter learned a ton about scientific technique, scientific methods, and about genetics, knockout models, UV damage, statistical analyses, and most importantly, science experiments don't always work right away! It takes a lot of work to get results in science.
Date published: 2019-03-16
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