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Description
Designed to match traditional AP® Biology Lab 6. It's easy to teach students the basics of DNA gel electrophoresis and analysis with this classic lab. Using convenient pre-cut DNA fragments, students electrophorese, stain, and visualize uncut lambda DNA (control) and DNA predigested with EcoRI and HindIII (standard molecular weight marker). They then determine the sizes of the DNA fragments. Enough materials for 8 teams of students.
Product Details
Beginning—Easy to perform, requires little or no prior knowledge.
The classic gel electrophoresis lab
Bring convenience to your gel electrophoresis instruction with the Restriction Enzyme Cleavage of DNA Kit, which features pre-cut DNA fragments and all reagents needed to get your students up and running a gel. Students determine the sizes for DNA fragments by performing electrophoresis on, staining, and visualizing an uncut lambda DNA control and DNA predigested with EcoRI and HindIII. The included dry labs provide additional supports for new learners and are perfect to use during gel running times.
Product Features
- Materials are sufficient for 8 student groups (8 gels)
- Load and go with aliquoted, precut, ready-to-use DNA samples
- Designed to match the traditional AP® Biology Lab 6
- Contains 2 bonus dry labs: "DNA Goes to the Races" and "DNA Scissors"
- Easy-to-use teacher's manual and student guide
Time Requirements
Teacher prep: 15 min. Lab: 60–90 min.
Learning Objectives
Students will:
- Learn about restriction enzymes and the analysis of restriction enzyme digests.
- Perform electrophoresis, a basic technique for separating DNA fragments by size.
- Use math skills including graphing on semi-log graph paper and interpolation.
AP® is a trademark registered and/or owned by the College Board®, which was not involved in the production of, and does not endorse, this product.
